Results: Migration Behavior of Bcl11a Mutant Upper-layer Cortical Neurons
6:40
Vibratome Sectioning and Slice Culture
4:18
Brain Dissection and Embedding for Organotypic Slice Culture
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Ime-lapse Imaging
Transcript
The overall goal of this procedure is to directly observe radially migrating neurons in organotypic slice culture prepared from electroporated embryonic brain by time-lapse confocal microscopy. This method can help study key aspects of neocortex d
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Denne protokol indeholder instruktioner til direkte observation af radialt migrerende kortikale neuroner. I utero- elektroporering, organotypisk skivekultur og time-lapse konfokal billeddannelse kombineres for direkte og dynamisk undersøgelse af virkningerne af overekspression eller nedregulering af gener af interesse for migrerende neuroner og for at analysere deres differentiering under udvikling.