Collection and Concentration of Viruses Expressing Metastatic Factor
2:26
Viral Titer Determination
4:37
Intracardiac Injection
7:24
Results: Detection of Metastatic Pancreatic β-cells by Immunostaining
8:16
Conclusion
Transcript
The overall goal of this procedure is to identify and characterize metastatic factors by gene transfer into the Novel RIP-Tag;RIP-tva mouse model. This method can help answer key questions in the cancer metastasis field such as identifying the dri
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Presentamos un protocolo para demostrar un sistema de transferencia génica somática novela utilizando RIP-Tag; RIP-tva modelo de ratón para estudiar la función de genes en la metástasis. El retrovirus aviares se entregan intracardiacally para asegurar la transferencia de genes en las lesiones premalignas, no invasivas de las células β pancreáticas en ratones adultos.