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Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

DOI :

10.3791/55903-v

June 16th, 2017

June 16th, 2017

11,960 Views

1Cell Biology and Neuroscience, University of California, Riverside

Recent advances in the ability to genetically manipulate somatic cell lines hold great potential for basic and applied research. Here, we present two approaches for CRISPR/Cas9 generated knockout production and screening in mammalian cell lines, with and without the use of selectable markers.

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