Results: Knockout Cell Lines Generated by Two CRISPER/Cas9 Methods
2:31
Isolation of Clonal Populations
0:57
Transfection of CRISPER Component into Cultured Cells and Drug Selection
3:53
Screening Candidates by Dot Blot
6:30
Screening Candidates by Colony PCR
Transcript
The goal of this procedure is to isolate and identify clonal knockout cell lines generated by two types of CRIPSR/Cas9-mediated genome editing. One method detects protein knockout caused by nonhomologous end joining while the other detects genomic
Sign in or start your free trial to access this content
I recenti progressi nella capacità di manipolare geneticamente le linee cellulari somatiche hanno un grande potenziale per la ricerca di base e applicata. Qui presentiamo due approcci per la produzione e lo screening generati da CRISPR / Cas9 nelle linee cellulari mammiferi, con e senza l'uso di marcatori selectable.