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DOI :
10.3791/55918-v
July 26th, 2017
Chapters
0:05
Title
1:07
Dissociation Protocol
2:42
Antibody Staining and Cell Sorting
4:39
Transfection
5:57
Ca2+ Imaging in Differentiated Cells and Image Analysis
8:50
Results: Myoblast Characterization and a SOCE Experiment
9:54
Conclusion
在这里,我们描述了一种从成年肌肉组织中获得纯种人成肌细胞的方法。这些细胞用于研究体外骨骼肌分化,特别是研究参与Ca 2+信号传导的蛋白质。
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