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DOI :
10.3791/55918-v
July 26th, 2017
Chapters
0:05
Title
1:07
Dissociation Protocol
2:42
Antibody Staining and Cell Sorting
4:39
Transfection
5:57
Ca2+ Imaging in Differentiated Cells and Image Analysis
8:50
Results: Myoblast Characterization and a SOCE Experiment
9:54
Conclusion
ここでは、成人の筋肉組織からのヒト筋芽細胞の純粋な集団を得るための方法を説明する。これらの細胞は、in vitro骨格筋分化を研究するため、特にCa 2+シグナル伝達に関与するタンパク質を研究するために使用される。
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