Name: cardiopulmonary bypass in a mouse model: a novel approach
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alle dyreforsøg blev udført i overensstemmelse med den tyske lov om dyr (TierSchG) og blev godkendt af den lokale dyrevelfærd (lavere Sachsen statslige kontor for forbrugerbeskyttelse og fødevaresikkerhed, protokol TSA 14/1556). Minimal vægten af musen egnet til denne model er 25 g.
 1. Præoperative præparater Bemærk: alle procedurer er udført under rene, ikke-sterile forhold, med autoklaveres instrumenter.
<ol><li>Sted 50-60 8-cm lang propylen ...

<ol>
	<li>Edmunds, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cardiopulmonary+Bypass+after+50+Years.">Cardiopulmonary Bypass after 50 Years.</a> N. Engl. J. Med. 351 (16), 1601-1603 (2004).</li><li>Goto, T., Maekawa, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cerebral+dysfunction+after+coronary+artery+bypass+surgery.">Cerebral dysfunction after coronary artery bypass surgery.</a> J. Anesth. 28 (2), 242-248 (2014).</li><li>Uysal, S., Reich, D. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neurocognitive+outcomes+of+cardiac+surgery.">Neurocognitive outcomes of cardiac surgery.</a> J. Cardiothorac. Vasc. Anesth. 27 (5), 958-971 (2013).</li><li>Ballaux, P. K., Gourlay, T., Ratnatunga, C. P., Taylor, K. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+literature+review+of+cardiopulmonary+bypass+models+for+rats.">A literature review of cardiopulmonary bypass models for rats.</a> Perfusion. 14 (6), 411-417 (1999).</li><li>Jungwirth, B., de Lange, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Animal+models+of+cardiopulmonary+bypass:+development,+applications,+and+impact.">Animal models of cardiopulmonary bypass: development, applications, and impact.</a> Semin. Cardiothorac. Vasc. 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Vi har udviklet en fuldt fungerende klinisk relevante model af CPB i en mus. Med mere end tredive stammer af mus har hjerte-kar-sygdomme, kunne vores model være et udgangspunkt for udviklingen af nye potentielle protokoller relateret til CPB. Desuden, på grund af overfloden af mus-specifikke reagenser og knockout-out mus, denne model kan ikke kun erstatte den nuværende rotte model af CPB men vil lette dissektion af de molekylære mekanismer involveret i CPB-relaterede organskader. Til dato har CPB ikke er anvendt i mu...

Siden indførelsen af hjertemassage bypass (CPB) ind på klinikken, har det spillet en væsentlig rolle i hjertekirurgi1. I moderne hjertekirurgi er langvarig CPB tid afgørende for at udføre omfattende aorta rekonstruktioner og kombinerede procedurer. Selv om teknologiske fremskridt har været enorm, brug af extracorporal omsætning er forbundet med intra - og postoperativ systemiske og lokale orgel skade2,3.
Store dyremodeller er blevet udviklet for at undersøge rollen som CPB fysiologiske processer4,5. Selv om disse modeller har givet indblik i nogle af CPB forbundet komplikationer, de er ekstremt dyrt og molekylære værktøjer (f.eks. antistoffer) er meget begrænset. Et mere omkostningseffektivt alternativ er blevet udviklet i små dyr. Siden deres udvikling, er flere undersøgelser blevet gennemført for at optimere en CPB model i rotter og kaniner5,6,7,8,9. Disse modeller er et godt grundlag for målinger af patofysiologiske sygdomsprocesser; de er imidlertid stadig utilstrækkelige til at undersøge cellulære og humorale immunologi på grund af manglende relevante antistoffer og reagenser. Dette forringer deres rolle inden for forskning.
Vi har for nylig udviklet en musemodel af CPB. På grund af en bred vifte af mus-specifikke reagenser og genmodificerede mus er musemodeller generelt model valg for fysiologiske, molekylære og immunologiske forskning10,11. Derfor vil vores model lette undersøgelse af CPB i forhold til forskellige co-morbiditet, som der er mange mus stammer tilgængelige med klinisk relevante sygdomme12,13. Dette papir beskriver i overensstemmelse hermed, i detaljer, hvordan man udfører CPB i mus. Ilt og hæmodynamiske parametre overvåges nøje efter dybt respiratorisk og cirkulatorisk anholdelse.

<table border="0" cellpadding="0" cellspacing="0" width="1191">
	<tbody>
		<tr height="23">
			<td height="23" style="height:23px;width:297px;">Sterofundin</td>
			<td style="width:279px;">B.Braun Petzold GmbH</td>
			<td style="width:181px;">PZN:8609189</td>
			<td style="width:435px;">priming volume, 1:1 with Tetraspan</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:297px;">Tetraspan 6% HES Solution</td>
			<td style="width:279px;">B. Braun Melsungen AG</td>
			<td style="width:181px;">PZN: 05565416</td>
			<td>priming volume, 1:1 with Sterofundin</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:297px;">Heparin Natrium 25.000</td>
			<td style="width:279px;">Ratiopharm GmbH</td>
			<td style="width:181px;">PZN: 3029843</td>
			<td>2.5 IU per ml of priming solution</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:297px;">NaHCO3 8,4% Solution</td>
			<td style="width:279px;">B. Braun Melsungen AG</td>
			<td style="width:181px;">PZN: 1579775</td>
			<td>3% in priming solution</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:297px;">KCL 7,45 % Solution</td>
			<td style="width:279px;">B. Braun Melsungen AG</td>
			<td style="width:181px;">PZN: 2418577</td>
			<td>0.1 ml for cardioplegia</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:297px;">Carprofen</td>
			<td style="width:279px;">Zoetis Inc., USA</td>
			<td>PZN:00289615 08859153</td>
			<td>5 mg/kg/BW</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:297px;">1 Fr PU Catheter</td>
			<td style="width:279px;">Instechlabs INC., USA</td>
			<td style="width:181px;">C10PU-MCA1301</td>
			<td>carotid artery</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:297px;">2 Fr PU Catheter</td>
			<td style="width:279px;">Instechlabs INC., USA</td>
			<td style="width:181px;">C20PU-MJV1302</td>
			<td>jugular vein</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Vasofix Safety catheter 20G</td>
			<td style="width:279px;">B.Braun Medical</td>
			<td style="width:181px;">4268113S-01</td>
			<td>orotracheal intubation</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:297px;">8-0 Silk suture braided</td>
			<td>Ashaway Line &#38; Twine Mfg. Co., USA</td>
			<td style="width:181px;">75290</td>
			<td>ligature</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:297px;">Isoflurane</td>
			<td style="width:279px;">Piramal Critical Care Deutschland GmbH</td>
			<td style="width:181px;">PZN:9714675</td>
			<td>narcosis</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">CLINITUBES blood capillaries</td>
			<td style="width:279px;">Radiomed GmbH</td>
			<td style="width:181px;">51750132</td>
			<td>blood sampling 60 - 95 microliter</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Spring Scissors - 6mm Blades</td>
			<td>Fine Science Tools GmbH</td>
			<td style="width:181px;">15020-15</td>
			<td>instruments</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Spring Scissors - 2mm Blades</td>
			<td>Fine Science Tools GmbH</td>
			<td style="width:181px;">15000-03</td>
			<td>instruments</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Halsted-Mosquito Hemostat</td>
			<td>Fine Science Tools GmbH</td>
			<td style="width:181px;">13009-12</td>
			<td>instruments</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Dumont #55 Forceps</td>
			<td>Fine Science Tools GmbH</td>
			<td style="width:181px;">11295-51</td>
			<td>instruments</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Castroviejo Micro Needle Holder - 9cm</td>
			<td>Fine Science Tools GmbH</td>
			<td style="width:181px;">12060-02</td>
			<td>instruments</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Micro Serrefines</td>
			<td>Fine Science Tools GmbH</td>
			<td>18555-01</td>
			<td>instruments</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Bulldog Serrefine</td>
			<td>Fine Science Tools GmbH</td>
			<td style="width:181px;">18050-28</td>
			<td>instruments</td>
		</tr>
		<tr height="23">
			<td height="23" style="height:23px;width:297px;">MiniVent Ventilator for Mice (Model 845)</td>
			<td style="width:279px;">Harvard Apparatus</td>
			<td style="width:181px;">73-0044</td>
			<td>mechanical ventilation</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:297px;">Isoflurane Vaporizer Drager 19.1</td>
			<td style="width:279px;">Dr&#228;gerwerk AG &#38; Co. KGaA</td>
			<td style="width:181px;"></td>
			<td>anesthesia 1.3 -2.5%</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">PowerLab data acquisition device 4/35</td>
			<td>ADInstruments Ltd, New Zealand</td>
			<td style="width:181px;">PL3504</td>
			<td>invasive pressure, ECG, temperature</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">ABL 800 Flex</td>
			<td>Radiometer GmbH</td>
			<td style="width:181px;"></td>
			<td>blood gas analysis</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:297px;">NMRI mice</td>
			<td style="width:279px;">Charles River Laboratories</td>
			<td style="width:181px;">Crl:NMRI(Han)</td>
			<td>male, 30-35 g, 12 weeks old, housed at least 1 week before the experiment</td>
		</tr>
	</tbody>
</table>

cardiopulmonary bypass in a mouse model: a novel approach

Som langvarig hjertemassage bypass bliver mere afgørende under hjerte interventioner, opstår en stigende kliniske behov for procedure optimering og for at minimere organskade som følge af langvarig extracorporal cirkulation. Målet med dette papir var at vise en fuldt funktionel og klinisk relevante model af hjertemassage bypass i en mus. Vi rapport om enhed design, perfusion kredsløb optimering og microsurgical teknikker. Denne model er en akut model, som ikke er kompatibel med overlevelse på grund af behovet for flere blod tegninger. På grund af vifte af værktøjer til rådighed for mus (fx, markører, knockouts, etc.), vil denne model lette undersøgelse af de molekylære mekanismer af organskader og effekten af hjertemassage bypass i forhold til andre co-morbiditet.

Denne protokol beskriver perfusion kredsløb, kirurgiske procedurer, og overvågning af fysiologiske parametre under CPB musen. Når der udføres af en tilstrækkeligt kvalificeret microsurgeon, er konsekvent og reproducerbar resultaterne.
For at opretholde passende væv perfusion, holdes den middel-arterielt tryk altid mellem 40 og 60 mmHg ved at justere CPB blodgennemstrømningen og tilføjelse af ekstra volumen. Afhængigt af...

Watch this Scientific Journal Video about Cardiopulmonary Bypass in a Mouse Model: A Novel Approach at JoVE.com

Cardiopulmonary Bypass in a Mouse Model: A Novel Approach

Dette papir beskriver hvordan man udfører hjertemassage bypass i mus. Denne nye model vil lette undersøgelsen af de molekylære mekanismer involveret i organskader.

Hjertemassage Bypass i en musemodel: en ny tilgang

As prolonged cardiopulmonary bypass becomes more essential during cardiac interventions, an increasing clinical demand arises for procedure optimization ...

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