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JoVE Journal

Medicine

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Laser Doppler: A Tool for Measuring Pancreatic Islet Microvascular Vasomotion In Vivo

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10:39 min

March 8th, 2018

March 8th, 2018

8,177 Views

0:04

Title

1:49

Using Streptozotocin and Sugar-water to Make Mice Diabetic

3:12

Preparing and Calibrating the Laser Doppler

4:24

Laser Doppler Analysis of Microvascular Vasomotion in Pancreatic Islet

7:56

Results: Microvascular Vasomotion in the Pancreatic Islet of STZ-induced Diabetic Mice

9:11

Conclusion

Transcript

The overall goal of this procedure is to determine the functional status of pancreatic islet microvascular vasomotion in vivo to assess its impact on related diseases. This method can help answer the key questions in the medical circulatory field

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Pancreatic islet microvascular vasomotion regulates islet blood distribution and maintains the physiological function of islet β cells. This protocol describes using a laser Doppler monitor to determine the functional status of pancreatic islet microvascular vasomotion in vivo and to assess the contributions of pancreatic islet microcirculation to pancreatic-related diseases.

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