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Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus

DOI :

10.3791/56035-v

February 1st, 2018

February 1st, 2018

8,202 Views

1Department of Developmental and Cell Biology, University of California, Irvine

Genes essential for survival pose technical hurdles for creating mutant lines. Leapfrogging circumvents lethality by combining genome editing with primordial germ cell transplantation to create wild-type animals carrying germline mutations. Leapfrogging also permits the efficient generation of homozygous null mutants in the F1 generation. Here, the transplantation step is demonstrated.

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