Generation of Lentiviral Transcriptional Reporter Cell Lines
3:47
Subclone Selection, Expansion, and Validation
4:47
Determination of GFAP:GFP Expression by Flow Cytometry
5:58
ELDA Self-renewal Assay to Assess Clonogenic Capacity
7:01
Drug Screen
8:27
Results: Selection and Drug Screening of Glioblastoma Stem Cell Subclones
9:46
Conclusion
Transcript
The overall goal of this procedure is to screen small molecule libraries for agents that promote astroglial differentiation in glioblastoma stem cells. This method can help answer key questions in the cancer stem cell field, such as what are the s
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En effektiv screening protokoll er presentert for identifikasjon av små molekyler som fremmer astroglial differensiering i glioblastom stilk celler (GSCs). Analysen er basert på en stilk cellen differensiering reporter der uttrykket av den forbedrede GFP (eGFP) er drevet av menneskelig GFAP selskapet.