Preparation of Eggs and Microdissection of Chicken Spinal Neural Tube (SNT)
4:12
Crosslinking Proteins to DNA
5:01
Lysis, Sonication, and Immunoprecipitation
6:33
Washing, Elution, and Reversal of Crosslinking
8:57
Cellular Protein and RNA Digestion
10:06
Results: Evaluation of the ChIP Protocol by ChIP-seq Experiments
11:48
Conclusion
Transcript
The overall goal of this procedure is to investigate the binding profiles of different histone modifications, using low-abundance embryonic samples in order to improve the functional annotation of vertebrate genomes. This method can help answer ke
Sign in or start your free trial to access this content
Aquí, describimos una inmunoprecipitación de cromatina (ChIP) y el protocolo de preparación de biblioteca de ChIP-seq para generar perfiles de epigenómica global de muestras embrionarias de pollo baja abundancia.