Deprotection and Purification of RNA Oligonucleotides
7:12
Characterization via MALDI-TOF and RNA Structure Analysis vi CD
9:24
Results: Characterization of RNA Oligomers Containing 2'-Othiophenylmethyl Modification
10:25
Conclusion
Transcript
The overall goal of this report is to illustrate the procedure to obtain oligonucleotides of RNA via solid-phase synthesis. Dodecamers of RNA will be synthesized as models and their purification and characterization will also be demonstrated. Thro
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Dit artikel geeft een gedetailleerde procedure voor de solide fase synthese, zuivering en karakterisering van dodecamers van RNA gemodificeerd op de C2'- O- positie. UV-vis en cirkelvormige dichroïstische fotometrische analyses worden gebruikt om structurele aspecten te kwantificeren en karakteriseren, dwz enkelstrengen of dubbelstrengen.