Quantitative Fluorescence Analysis of Immunostained or Live Cells
6:54
Cell Cycle and G1 Phase Synchronization
7:46
Results: In Vivo Cellular Responses to Complex DNA Damage
9:27
Conclusion
Transcript
The overall goal of this protocol is to describe how to use laser microirradiation to induce different types of DNA damage, including simple strand breaks, and complex damage, to study DNA damage signaling and repair factor assembly in vivo. In th
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该协议的目的是描述如何使用激光 microirradiation 诱导不同类型的 dna 损伤, 包括相对简单的断链和复杂的损害, 研究 dna 损伤信号和修复因子在损伤部位的组装在体内.