Quantitative Fluorescence Analysis of Immunostained or Live Cells
6:54
Cell Cycle and G1 Phase Synchronization
7:46
Results: In Vivo Cellular Responses to Complex DNA Damage
9:27
Conclusion
Transcript
The overall goal of this protocol is to describe how to use laser microirradiation to induce different types of DNA damage, including simple strand breaks, and complex damage, to study DNA damage signaling and repair factor assembly in vivo. In th
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Het doel van dit protocol is te beschrijven hoe laser microirradiation voor het opwekken van verschillende soorten DNA-beschadiging, met inbegrip van relatief eenvoudige strand einden en complexe schade, aan de studie van DNA schade signalering en reparatie factor montage op schade sites in vivo .