Quantitative Fluorescence Analysis of Immunostained or Live Cells
6:54
Cell Cycle and G1 Phase Synchronization
7:46
Results: In Vivo Cellular Responses to Complex DNA Damage
9:27
Conclusion
Transcript
The overall goal of this protocol is to describe how to use laser microirradiation to induce different types of DNA damage, including simple strand breaks, and complex damage, to study DNA damage signaling and repair factor assembly in vivo. In th
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O objetivo do presente protocolo é descrever como usar o microirradiation do laser para induzir diferentes tipos de danos no DNA, incluindo relativamente simples quebras e danos complexos, para estudar o DNA danos reparação e sinalização fator montagem em locais de dano in vivo .