Name: touchscreen sustained attention task (sat) for rats
Uploaded: 2017-09-15T15:00:00
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土パラメーターに喜びベルクマン、アッティリオスリック Ceretti、サラ ・ コーエン、ニーナ ダンカン、アーロン ホール、ハンナ Lefebo と動作で、援助のマドレーヌ サルヴァトーレと博士ヴィネ パリキ、博士ブリトニー · Yegla とロブ ・ コールが彼らのアドバイスに感謝したいと思います。トレーニング手順に関するヒント博士ジル McGaughy に感謝したいと思います。この仕事は健康補助金ニッケル水素 092438 研究機構によって支えられた、全米科学財団は、D.A.B. に IOS 1552416 を付与

すべての実験健康の国民の協会のガイドラインに従って実施し、寺院大学機関動物使用とケア委員会によって承認されました。このプロトコルは大人で開発された (~ 60 日齢研究開始時) 男性と女性の Sprague-dawley ラット (チャールズ川).
 1 タッチ スクリーン土材料 <ol><li>ことを確認音の減衰のブースがそれぞれのタッチ スクリーン室を囲むと家光、音発生器、カメラ、?...

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神経の持続的注意の障害が報告される、神経発達、および神経変性疾患1,2,3、対混乱注意最適に貢献するプロセスがけいこ。これは、一部では、特殊な装置齧歯動物注意課題の簡単な適応を制限するためです。ただし、タッチ スクリーン室機器20,22の 1 枚でさまざまな認知タス?...

共有機能1,2,3注意障害の疾患アルツハイマー病、統合失調症、注意欠陥多動性障害 (ADHD) に至る。持続的注意の障害、断続的で予測不可能なイベントの状況を継続的に監視する機能 — 持続的注意は選択と分割の注意と同様、他の認知のために重大、特に破壊的な4,5を処理します。健康な人でさえ否定的注意の維持の難しさ損なう毎日関数6認知に影響します。したがって、持続的な関心とどのようにそれはなる調節不全の神経生物学の基礎の理解につながる介入多くの人々 の利益となる認知能力を改善します。
回路と対混乱注意適切に貢献する分子プロセスを記述するために多くの研究者がヒト以外の動物のモデルになっている、注目中の特定の細胞および分子プロセスの操作タスクは可能です。努力は、さまざまな注目7,8,9,10を維持する能力を評価することがオペラント注意タスクの開発につながっています。持続的注意タスクとして知られている McGaughy と単 (1995 年) によって開発されたそのようなの 1 つのパラダイム (土)。SAT の訓練を受けての齧歯動物は、非信号試験からの光信号は簡潔に点滅している信号試験を区別する必要があります。注意力の需要は、視覚妨害 (点滅 houselight)10を導入することで増やすことが。土に注意を査定するための重要なパラメーターはよく文書化され、このタスクが検証された雄および雌ラットおよびマウス10、11,12。バージョンも土13の並進運動の有用性を強調、人間に適応されています。重要なは、このタスクを使用する持続的注意4前脳基底部の corticopetal システムを巻き込むできました。マイネルト核 (NBM) ので具体的には、コリン作動性ニューロンの信号試験14に正確な応答をヒット前脳基底部前頭前野にプロジェクトの質 inominata (SI) 地域に欠かせない/ 15。対照的に、この地域における gaba 作動性ニューロンは非信号試験16正確な応答である正しい拒絶 (CRs) 時のパフォーマンスを仲介すると考えられています。このタスクの基本的な回路が確立されると、ストレス ホルモンから神経毒性タンパク質に至るまで注目を損なうこの回路の変調の要因は識別された17,18をされています。総称して、これらの研究は、土の有用性を強調表示します。
研究室では、土を実装する 1 つの制限は、元のプロシージャがセンター パネル ライトと 2 つの拡張可能なレバー、ヒットと CRs10の 1 つのための指定、タスクの完全に構成されているオペラントの部屋を必要とすることです。信号試験光のパネルは簡単に点灯し、レバーを 4 s 応答ウィンドウを示す拡張します。その期間中に指定されたヒット レバーが押された場合、ラット報酬を受ける (食糧または水)。信号試験、ミス、として知られている不適切なレバー押しは報われない。非シグナル状態試験、パネルのライトが消灯し、レバーを拡張します。CR が報われる一方、非シグナル状態試験、誤報、として知られている不適切なレバー押しは報われない。任意の応答を応答ウィンドウ中にする失敗は、不作為としてカウントされます。土の可用性を展開すると、最近 1 つの壁、両方の視覚刺激とレコード ノーズポーク反応19を表示できるタッチ スクリーン、タッチ スクリーン オペラント チャンバーズのタスクを変更しました。タッチ スクリーンの部屋は装置20の 1 枚で、さまざまなタスク (例えば、ペアに関連付ける学習、逆転学習など) を実行する汎用性のために人気になっています。他のタッチ スクリーンのプロシージャに似ています20,21,22, 我々 の適応土の穴にそれをカット、マスクと呼ばれる、不透明なプラスチック製のタッチパネル カバーの使用が必要です。背後にある、画面に白い刺激を提示することによって信号のプレゼンテーションは、中央の円形のホールと 2 つの方形波の応答領域を 1 つのヒットと CRs、指定できる応答19に触れるようにラット。伝統的な部屋で 4 s の応答ウィンドウは、通常、レバーのプレゼンテーションで示されますが、以前の研究実証トーンは信号の応答ウィンドウ23にも使用できます。タッチ スクリーンの土で同様に短音の応答ウィンドウを信号します。他のトレーニングのパラメーター (例えば試験、試行間間隔等の数) は伝統的な間で類似保持されますとタッチ スクリーン (土) です。我々 は以前伝統とタッチ スクリーンの SAT バージョン間のパフォーマンスを評価および SAT は持続的注意19を測定する有効な方法で対等だったが見つかりました、そのタッチ スクリーンを示唆しています。タッチ スクリーンにより、土より多目的なのでそれは今タッチ スクリーン オペラント ボックスの広まった使用のために適応することができます。
現在のプロトコルの詳細をどのようにタッチ スクリーンを実行する研究室では、土。トレーニング スケジュールは、雌のラットは、男性とは違ってあった前スケジュール24を進める難易度で獲得時間を最適化する作業19の前報から若干変更されています。雄および雌ラットの典型的なパフォーマンスを示す結果は、タスクの問題のトラブルシューティングのためのヒントと一緒に、含まれています。

<table>
	<tbody>
		<tr>
			<td>Bussey-Saksida Rat Touch Screen Chambers Easy-Install System</td>
			<td>Lafayette Instrument</td>
			<td>80604-20</td>
			<td>Includes: Touch Screen, Chamber base with perforated floors, Large Feeder Reward Area (44.5mm wide x 92.5mm high), Speaker, Houselight, Tone Generator, SAC; Trapezoidal animal working area is 126mm wide at the Feeder, 240mm wide and 332 mm deep at the Screen, and 300 mm in height</td>
		</tr>
		<tr>
			<td>Sound Attenuation Cubicles (SAC)</td>
			<td>Lafayette Instrument</td>
			<td>80604-20</td>
			<td>Attenuation to approximately 35dB; 38kg in weight; External dimensions: 600mm wide x 670mm tall x 352mm deep; Internal dimensions: 540mm wide x 610mm tall x 532mm deep</td>
		</tr>
		<tr>
			<td>Abet II Software for Touch Screens</td>
			<td>Lafayette Instrument</td>
			<td>89505</td>
			<td>Version 2.19</td>
		</tr>
		<tr>
			<td>Whisker Multimedia</td>
			<td>Lafayette Instrument</td>
			<td>80698-1</td>
			<td>Required for Abet II Touch Operation</td>
		</tr>
		<tr>
			<td>Pellet Dispenser</td>
			<td>Lafayette Instrument</td>
			<td>80209-45</td>
			<td>45mg Interchangeable Pellet Size Wheel</td>
		</tr>
		<tr>
			<td>Camera</td>
			<td>Lafayette Instrument</td>
			<td>80600-CAM</td>
			<td>Filtered and focused for IR light</td>
		</tr>
		<tr>
			<td>Microsoft Windows</td>
			<td>Microsoft</td>
			<td></td>
			<td>Windows 7-64 bit recommended</td>
		</tr>
		<tr>
			<td>Controller PC-Touch Screen Chambers</td>
			<td>Lafayette Instrument</td>
			<td>88530</td>
			<td>Dual Core Pentinum Processor; 2.5 GHz or greater; keyboard, mouse, and monitor; Installed PCI card, cable and expansion; Four RS232 ports; Four VGA ports</td>
		</tr>
		<tr>
			<td>Dustless Precision Pellets</td>
			<td>BioServe</td>
			<td>F0165</td>
			<td>45 mg</td>
		</tr>
		<tr>
			<td>Black Acrylic Mask</td>
			<td>Everything Plastic</td>
			<td>Custom Product</td>
			<td>Black polycarbonate with one matte finish side, 3.18 mm thick; Central circular opening, 28.58 mm diameter, 107.95 mm from the bottom, centered between the left and right side; Two square response areas, 28.57 mm x 28.57mm each positioned below and off center, one 85.725 mm from the left and one 85.725 mm from right side, and both 34.925 mm from the bottom.</td>
		</tr>
		<tr>
			<td>Utility Cart</td>
			<td>Fisher Scientific</td>
			<td>11-954-754</td>
			<td>30.75 in. x 18.38 in x 33 in</td>
		</tr>
		<tr>
			<td>Black towel</td>
			<td></td>
			<td></td>
			<td>Large enough to cover cages for animal transport</td>
		</tr>
		<tr>
			<td>Headlights with red lights</td>
			<td>Energizer</td>
			<td>HDL33A2E</td>
			<td>LED white and red light</td>
		</tr>
	</tbody>
</table>

touchscreen sustained attention task (sat) for rats

持続的な関心は、長期間にわたって断続的に予測不可能なイベントを監視する機能です。この注意プロセス認知の他の側面を subserves し、特定の神経発達、神経で破壊されると神経変性疾患。したがってを損なうし、持続的な関心を向上させるメカニズムを識別するために臨床的に重要です。そのようなメカニズムは頻繁に最初齧歯動物モデルを使用して発見しました。したがって、持続的注意の側面をテストするためのいくつかの動作手順は、齧歯動物のために開発されています。McGaughy と単 (1995 年) で最初に説明した、1 つは持続的注意タスクと呼ばれる (土) 信号 (すなわち、簡単な光のプレゼンテーション) と非信号試験区別するラットの列車します。信号は短く、こうして感知されるに細心の注意が必要です。注意力の需要を増やすことができます (例えばhouselight の点滅) ディス トラクターを導入することによってさらに。このタッチ スクリーン オペラントのチェンバースは、刺激と応答を記録を提示することができます 1 つの壁にタッチ スクリーンが設定されているタスクを変更しました。ここでは、タッチ スクリーンの部屋で座っていたために、我々 のプロトコルを詳しく説明します。さらに、男性と女性の Sprague-dawley ラットのパフォーマンスの標準的な措置を提案します。男女ともにこのタスクに匹敵する性能は、多くの研究者が、実験的なデザインで女性の齧歯動物を含む、特に注意研究のための使用を強調表示します。さらに、ますます人気のあるタッチ スクリーン室土の簡単な実装は、その有用性を増加します。

ほとんどの注意研究のラットは、土の条件に訓練を受けています、破壊または注意を改善する操作が実行します。集録データは通常表示されない、雌雄ラットにおける獲得率を説明するためにここで 。このため、土 (鼻を突くフェーズの開始) の基準に到達する日の数の中央値を定量化しました。いくつかのラット絶対加えそれ条件 (n = 5 の男性と n = 1 の女性)。男性?...

Watch this Scientific Journal Video about Touchscreen Sustained Attention Task SAT for Rats at JoVE.com

Touchscreen Sustained Attention Task SAT for Rats

タッチ スクリーンの持続的注意課題 (土) ラット

持続的注意、または断続的な予測不可能なイベントの状況を継続的に監視は、認知の重要な側面です。ここでタッチ スクリーン オペラント チャンバーを用いたラットでの持続的な関心をテストする方法を詳しく説明します。我々 は男女ともに注意を調べる場合に役立ちますこのタスクを作る雄および雌ラットに匹敵する性能を発揮します。

Sustained attention is the ability to monitor intermittent and unpredictable events over a prolonged period of time. This attentional process subserves ...

The overall goal of this behavioral task with touchscreen operant chambers is to measure the sustained attention capacity of rats. This method can help uncover the neuro biological mechanisms critical for appropriate sustained attention which is the monitoring of intermittent and unpredictable events over time. The main advantage of this technique is that we have adapted a well validated rodent sustained attention task to the touchscreen operant chamber.An apparatus of growing popularity with programmability to many cognitive tasks. The applications of this technique extend toward the study of neuropsychiatric and neurodegenerative disorders characterized by disrupted attention. Therapies for which, can be tested using the method presented here.Generally, individuals new to this method will struggle with setting up a system to ensure that the rats are being accurately tracked and that the data is being properly recorded. For this experiment, touchscreen chambers must be prepared within sound attenuation cubicles. Equip the chambers with the house light, a tone generator, a camera positioned above, a touchscreen and a pellet dispenser with the feeder reward area.The feeder reward area can be illuminated. Before loading the 45 milligram reward pellets, shake the dust off them using a flour sifter. This dust can clog the feeder.If the rats are outfitted with a head implant, use a feeder with a large aperture. Feed each chamber with a black acrylic mask to limit the response areas on the touchscreen. Leave a central circular hole for the light stimulus and two square response holes below the central hole positioned center.While in use, operate the chamber in a sound attenuation cubicle. This completes the chamber preparations. The text protocol describes how to set the perimeters for the experiment.For this experiment, rats can be housed individually or in groups. Prior to starting a study, handle the rats daily for five minutes per day, for five straight days. Always wear personal protective gear.Start the food restriction three days before starting the behavior shaping. On the first day, record each rats free-feeding weight and calculate their target weight which is 85%of the free-feeding weight. Prior to shaping, pre-expose the rats to 15 food reward pellets in their home cage.After the initial food restriction, adjust the food intake to maintain the target weight, allowing for slight weight gain over time. Prior to starting a new schedule, food restrict the rats a little more than usual, to increase their motivation. On days that rats do not perform the task, increase their food by at least one gram to compensate for not receiving pellet rewards.After each session, wait at least 30 minutes before providing food, to avoid making an association between the testing succession and their feeding time. If the rats are group housed, feed them separately for an hour before regrouping them. In preparation, power up the equipment.Ensure that the pellet dispensers are filled with pellets and ensure that the mask is in position in front of the touchscreen. Then, check the function of each box. Test the pellet dispenser, the screen, the lights and the tone generator.Testing the function of the chambers at the beginning of each day is critical because any one small malfunction can confuse the animals and prevent them from learning the task or performing at a normal level. Next, review the schedule for loading the boxes. Pay close attention to the test group and input the relevant information regarding animal ID, sex, experimental condition, user initials and other notes.With all the preparations made, retrieve the subjects. Generally, test the rats at least 30 minutes into their dark cycle. To work with the rats in the dark, use head mounted red lights.When transporting the rats during the dark cycle, cover their cages with black towels. Once in the behavior room, load the rats into their appropriate box and start the session for the sustained attention task. In the sustained attention task, rats are trained to distinguish signal trials from non-signal trials.Signal trials include a variable duration light flash followed by an audible tone and a four second response window. During the four seconds, if the rat then posed the correct area on the screen. In this case, it is the right response area, the rat is rewarded with a food pellet and this is called, a hit.If the rat pokes the other response area, this is considered a miss and the rat is not rewarded. A non-signal trial does not include the light and the rat must poke a different part of the screen to indicate that no signal was detected to earn a reward. A correct response on a non-signal trial is called, a correct rejection.If the rat pokes the other response area on a non-signal trial, this is considered a false alarm and is not rewarded. The correct response areas for signal and non-signal trials should be counter-balanced between subjects. For signal and non-signal trials, a failure to make any response during the response window counts as a, no mission and is not rewarded.There is also a distracter version of the sustained attention task, where the house light flashes during the second of three blocks of trials. After starting a session, monitor the rats during the first several minutes, to ensure that everything is functioning properly. Then, check their performance intermittently.Once a session is complete, record the summary data and any issues in the notebook. Between trials, wipe down each chamber with 10%ethanol and replace the bedding. After running all the trials for the day, thoroughly clean the chambers with 10%ethanol, making sure the floor grid is as clean as possible.Then, replace the bedding. After every session, it is a good idea to fully update the spreadsheets and back them up to a separate server. The median number of days to reach criteria on SAT was 54.5 days for male rats and 62 days for female rats with no significant difference.Performance measures on the SAT task showed that based on the percentages of hits and correct rejections, males and females were similarly accurate on the signaled and non-signaled trials. An overall measure of intentional performance, the vigilance index or VI, showed similar measures between the sexes as well. Nor were there sex differences in the low percentages of omissions recorded.During the tasks, the visual signal varied between 500, 50 and 25 milliseconds and performance declined with the shorter signals. There was no main effect based on sex. To increase attentional demands, the house light was flashed as a distracter during the second of the three trial blocks, in one subset of rats.As expected, there was a main effect for the trial block. With performance declining in block two and recovering in block three. Unexpectedly, females performed significantly worse than males.After watching this video, you should have a good understanding of how to test sustained attention in rats using touchscreen operant chambers. While attempting this procedure, it's important to remember to keep track of each individual animal's performance and adjust feeding as necessary to ensure that they have acquired the task as quickly as possible. Don't forget, that when working with rodents, protocol should first be approved by IACUC and personal protective equipment should always be worn.

touchscreen-sustained-attention-task-sat-for-rats

Research

JoVE Journal

Neuroscience

Touchscreen Sustained Attention Task (SAT) for Rats

Correlating Behavioral Responses to fMRI Signals from Human Prefrontal Cortex: Examining Cognitive Processes Using Task Analysis

The aim of this methods paper is to describe how to implement a neuroimaging technique to examine complementary brain processes engaged by two similar tasks. Participants' behavior during task performance in an fMRI scanner can then be correlated to the brain activity using the blood-oxygen-level-dependent signal. We measure behavior to be able to sort correct trials, where the subject performed the task correctly and then be able to examine the brain signals related to correct performance. Conversely, if subjects do not perform the task correctly, and these trials are included in the same analysis with the correct trials we would introduce trials that were not only for correct performance. Thus, in many cases these errors can be used themselves to then correlate brain activity to them. We describe two complementary tasks that are used in our lab to examine the brain during suppression of an automatic responses: the stroop1 and anti-saccade tasks. The emotional stroop paradigm instructs participants to either report the superimposed emotional 'word' across the affective faces or the facial 'expressions' of the face stimuli1,2. When the word and the facial expression refer to different emotions, a conflict between what must be said and what is automatically read occurs. The participant has to resolve the conflict between two simultaneously competing processes of word reading and facial expression. Our urge to read out a word leads to strong 'stimulus-response (SR)' associations; hence inhibiting these strong SR's is difficult and participants are prone to making errors. Overcoming this conflict and directing attention away from the face or the word requires the subject to inhibit bottom up processes which typically directs attention to the more salient stimulus. Similarly, in the anti-saccade task3,4,5,6, where an instruction cue is used to direct only attention to a peripheral stimulus location but then the eye movement is made to the mirror opposite position. Yet again we measure behavior by recording the eye movements of participants which allows for the sorting of the behavioral responses into correct and error trials7 which then can be correlated to brain activity. Neuroimaging now allows researchers to measure different behaviors of correct and error trials that are indicative of different cognitive processes and pinpoint the different neural networks involved.

The goal of our research is to correlate behavior to brain activity. Accurate behavioral measures and imaging techniques allow us to elucidate brain-behavior relationships.

人間の前頭前野皮質からfMRI信号への行動応答の相関：タスク分析を用いた認知プロセスを調べる

The aim of this methods paper is to describe how to implement a neuroimaging technique to examine complementary brain processes engaged by two similar ...

神経科学

A General Method for Evaluating Incubation of Sucrose Craving in Rats

For someone on a food-restricted diet, food craving in response to food-paired cues may serve as a key behavioral transition point between abstinence and relapse to food taking 1. Food craving conceptualized in this way is akin to drug craving in response to drug-paired cues. A rich literature has been developed around understanding the behavioral and neurobiological determinants of drug craving; we and others have been focusing recently on translating techniques from basic addiction research to better understand addiction-like behaviors related to food 2-4.
As done in previous studies of drug craving, we examine sucrose craving behavior by utilizing a rat model of relapse. In this model, rats self-administer either drug or food in sessions over several days. In a session, lever responding delivers the reward along with a tone+light stimulus. Craving behavior is then operationally defined as responding in a subsequent session where the reward is not available. Rats will reliably respond for the tone+light stimulus, likely due to its acquired conditioned reinforcing properties 5. This behavior is sometimes referred to as sucrose seeking or cue reactivity. In the present discussion we will use the term "sucrose craving" to subsume both of these constructs.
In the past decade, we have focused on how the length of time following reward self-administration influences reward craving. Interestingly, rats increase responding for the reward-paired cue over the course of several weeks of a period of forced-abstinence. This "incubation of craving" is observed in rats that have self-administered either food or drugs of abuse 4,6. This time-dependent increase in craving we have identified in the animal model may have great potential relevance to human drug and food addiction behaviors.
Here we present a protocol for assessing incubation of sucrose craving in rats. Variants of the procedure will be indicated where craving is assessed as responding for a discrete sucrose-paired cue following extinction of lever pressing within the sucrose self-administration context (Extinction without cues) or as responding for sucrose-paired cues in a general extinction context (Extinction with cues).

Responding for food or drug-paired cues increases over the course of a period of abstinence and this may relate to an increased susceptibility to relapse behaviors. Here we detail a procedure for evaluating this "incubation of craving" in rats that have self-administered sucrose.

ラットのショ糖の渇望のインキュベーションを評価するための一般的な方法

For someone on a food-restricted diet, food craving in response to food-paired cues may serve as a key behavioral transition point between abstinence and ...

Mapping Cortical Dynamics Using Simultaneous MEG/EEG and Anatomically-constrained Minimum-norm Estimates: an Auditory Attention Example

Magneto- and electroencephalography (MEG/EEG) are neuroimaging techniques that provide a high temporal resolution particularly suitable to investigate the cortical networks involved in dynamical perceptual and cognitive tasks, such as attending to different sounds in a cocktail party. Many past studies have employed data recorded at the sensor level only, i.e., the magnetic fields or the electric potentials recorded outside and on the scalp, and have usually focused on activity that is time-locked to the stimulus presentation. This type of event-related field / potential analysis is particularly useful when there are only a small number of distinct dipolar patterns that can be isolated and identified in space and time. Alternatively, by utilizing anatomical information, these distinct field patterns can be localized as current sources on the cortex. However, for a more sustained response that may not be time-locked to a specific stimulus (e.g., in preparation for listening to one of the two simultaneously presented spoken digits based on the cued auditory feature) or may be distributed across multiple spatial locations unknown a priori, the recruitment of a distributed cortical network may not be adequately captured by using a limited number of focal sources.
Here, we describe a procedure that employs individual anatomical MRI data to establish a relationship between the sensor information and the dipole activation on the cortex through the use of minimum-norm estimates (MNE). This inverse imaging approach provides us a tool for distributed source analysis. For illustrative purposes, we will describe all procedures using FreeSurfer and MNE software, both freely available. We will summarize the MRI sequences and analysis steps required to produce a forward model that enables us to relate the expected field pattern caused by the dipoles distributed on the cortex onto the M/EEG sensors. Next, we will step through the necessary processes that facilitate us in denoising the sensor data from environmental and physiological contaminants. We will then outline the procedure for combining and mapping MEG/EEG sensor data onto the cortical space, thereby producing a family of time-series of cortical dipole activation on the brain surface (or "brain movies") related to each experimental condition. Finally, we will highlight a few statistical techniques that enable us to make scientific inference across a subject population (i.e., perform group-level analysis) based on a common cortical coordinate space.

We use magneto- and electroencephalography (MEG/EEG), combined with anatomical information captured by magnetic resonance imaging (MRI), to map the dynamics of the cortical network associated with auditory attention.

同時MEG / EEGおよび解剖学的に制約のある最小ノルム推定値を使用してマッピング皮質ダイナミクス：聴覚注意の例

Magneto- and electroencephalography (MEG/EEG) are neuroimaging techniques that provide a high temporal resolution particularly suitable to investigate the ...

Conducting Concurrent Electroencephalography and Functional Near-Infrared Spectroscopy Recordings with a Flanker Task

Concurrent EEG and fNIRS recordings offer an excellent opportunity to gain a full understanding of the neural mechanism of cognitive processing by inspecting the relationship between the neural and hemodynamic signals. EEG is an electrophysiological technology that can measure the rapid neuronal activity of the cortex, whereas fNIRS relies on the hemodynamic responses to infer brain activation. The combination of EEG and fNIRS neuroimaging techniques can identify more features and reveal more information associated with the functioning of the brain. In this protocol, fused EEG-fNIRS measurements were performed for concurrent recordings of evoked-electrical potentials and hemodynamic responses during a Flanker task. In addition, the critical steps for setting up the hardware and software system as well as the procedures for data acquisition and analysis were provided and discussed in detail. It is expected that the present protocol can pave a new avenue for improving the understanding of the neural mechanisms underlying various cognitive processes by using the EEG and fNIRS signals.

The present protocol describes how to perform concurrent EEG and fNIRS recordings and how to inspect the relationship between the EEG and fNIRS data.&#160;

フランカータスクによる同時脳波と機能的近赤外分光記録の実施

Concurrent EEG and fNIRS recordings offer an excellent opportunity to gain a full understanding of the neural mechanism of cognitive processing by ...

Event Related Potentials (ERPs) and other EEG Based Methods for Extracting Biomarkers of Brain Dysfunction: Examples from Pediatric Attention Deficit/Hyperactivity Disorder (ADHD)

Neuropsychiatric diagnoses like ADHD are based on subjective methods like interviews, rating scales and observations. There is a need for more brain-based supplements. Stimulant medication is the most common treatment for ADHD. Clinically useful predictors of response have so far not been reported. The aim of this paper is to describe the EEG based methods we apply to extract potential biomarkers for brain dysfunction. Examples relate to biomarkers for pediatric ADHD, and prediction of medication response. The main emphasis is on Event Related Potentials (ERPs).
A nineteen channel EEG is recorded during a 3 min eyes-opened task, a 3 min eyes-closed task, and a 20 min cued visual GO/NOGO task (VCPT). ERPs are recorded during this task. The goal of the ERP protocol is to extract biomarkers of assumed brain dysfunctions that significantly differentiate between a patient group and healthy controls. The protocol includes recording during standard conditions and artifact correction. ERP waves can be used or transformed into latent components. The components of the patient group are compared with controls, empathizing components that, when compared, show relatively high effect sizes. Sub-groups of the patients are selected on the basis of the cluster analysis in the space of the components. Treatment procedure (such as medication, tDCS or neurofeedback protocol) can be applied and the changes in components related to treatment in the subgroups are observed, forming the basis for clinical recommendations.
The methods described were applied in a study of 87 pediatric ADHD patients. The index of medication response discriminated significantly between responders and non-responders with a large, and clinically meaningful effect size (d = 1.84). In an ongoing study comparing ADHD children with matched controls, several variables discriminate significantly between patients and controls. The global index will exceed d = .8. The EEG based methods described here could be clinically meaningful.

Event Related Potentials ERPs and other EEG Based Methods for Extracting Biomarkers of Brain Dysfunction: Examples from Pediatric Attention Deficit/Hyperactivity Disorder ADHD

EEG-methods are applied for extracting biomarkers of brain dysfunctions. The focus is on multi-channel event-related potentials (ERPs) recorded in a cued GO/NOGO task. Non-brain artifacts are corrected and ERPs are compared with the normative data. Examples relate to biomarkers for ADHD diagnosis and prediction of medication response.

脳機能障害のバイオマーカーを抽出するためのイベント関連電位(ERP)および他の脳科学ベースの方法:小児注意欠陥/多動性障害(ADHD)の例

Neuropsychiatric diagnoses like ADHD are based on subjective methods like interviews, rating scales and observations. There is a need for more brain-based ...

Preparation of Peripheral Nerve Stimulation Electrodes for Chronic Implantation in Rats

Peripheral nerve cuff electrodes have long been used in the neurosciences and related fields for stimulation of, for example, vagus or sciatic nerves. Several recent studies have demonstrated the effectiveness of chronic VNS in enhancing central nervous system plasticity to improve motor rehabilitation, extinction learning, and sensory discrimination. Construction of chronically implantable devices for use in such studies is challenging due to rats&#8217; small size, and typical protocols require extensive training of personnel and time-consuming microfabrication methods. Alternatively, commercially available implantable cuff electrodes can be purchased at a significantly higher cost. In this protocol, we present a simple, low-cost method for construction of small, chronically implantable peripheral nerve cuff electrodes for use in rats. We validate the short and long-term reliability of our cuff electrodes by demonstrating that VNS in ketamine/xylazine anesthetized rats produces decreases in breathing rate consistent with activation of the Hering-Breuer reflex, both at the time of implantation and up to 10 weeks after device implantation. We further demonstrate the suitability of the cuff electrodes for use in chronic stimulation studies by pairing VNS with skilled lever press performance to induce motor cortical map plasticity.

Existing approaches for constructing chronically implantable peripheral nerve cuff electrodes for use in small rodents often require specialized equipment and/or highly trained personnel. In this protocol we demonstrate a simple, low-cost approach for fabricating chronically implantable cuff electrodes, and demonstrate their effectiveness for vagus nerve stimulation (VNS) in rats.

ラットの慢性移植のための末梢神経刺激電極の調製

Peripheral nerve cuff electrodes have long been used in the neurosciences and related fields for stimulation of, for example, vagus or sciatic nerves. ...

The Impact of Motor Task Conditions on Goal-Directed Arm Reaching Kinematics and Trunk Compensation in Chronic Stroke Survivors

Trunk compensation is the most common movement strategy to substitute for upper extremity (UE) motor deficits in chronic stroke survivors. There is a lack of evidence examining how task conditions impact trunk compensation and goal-directed arm reaching kinematics. This protocol aims to investigate the impact of task conditions, including task difficulty and complexity, on goal-directed arm reaching kinematics. Two non-disabled young adults and two chronic stroke survivors with mild UE motor impairment were recruited for testing the protocol. Each participant performed goal-directed arm reaches with four different task conditions (2 task difficulties [large vs. small targets] X 2 task complexities [pointing vs. picking up]). The task goal was to reach and point at a target or pick up an object located 20 cm in front of the home position as quickly as possible with a stylus or a pair of chopsticks, respectively, in response to an auditory cue. Participants performed ten reaches per task condition. A 3-dimensional motion capture camera system was used to record trunk and arm kinematics. Representative results showed that there was a significant increase in movement duration, movement jerkiness, and trunk compensation as a function of task complexity, but not task difficulty in all participants. Chronic stroke survivors showed significantly slower, jerkier, and more feedback-dependent arm reaches and significantly more compensatory trunk movements than non-disabled adults. Our representative results support that this protocol can be used to investigate the impact of task conditions on motor control strategies in chronic stroke survivors with mild UE motor impairment.

This protocol is intended to investigate the impact of task conditions on movement strategies in chronic stroke survivors. Further, this protocol can be used to examine if a restriction in elbow extension induced by neuromuscular electrical stimulation causes trunk compensation during goal-directed arm reaches in non-disabled adults.

慢性脳卒中生存者における運動学とトランク補償に到達する目標指向腕に及ぼす運動タスク条件の影響

Trunk compensation is the most common movement strategy to substitute for upper extremity (UE) motor deficits in chronic stroke survivors. There is a lack ...

Head Implants for the Neuroimaging of Awake, Head-Fixed Rats

Anesthetics, commonly used in preclinical and fundamental scientific research, have a depressive influence on the metabolic, neuronal, and vascular functions of the brain and can adversely influence neurophysiological results. The use of awake animals for research studies is advantageous but poses the major challenge of keeping the animals calm and stationary to minimize motion artifacts throughout data acquisition. Awake imaging in smaller-sized rodents (e.g., mice) is very common but remains scant in rats as rats are bigger, stronger, and have a greater tendency to oppose movement restraints and head fixation over the long durations required for imaging. A new model of neuroimaging of awake, head-fixed rats using customized hand-sewn slings, 3D-printed head implants, head caps, and a headframe is described. The results acquired following a single trial of single-whisker stimulation suggest an increase in the intensity of the evoked functional response. The acquisition of the evoked functional response from awake, head-fixed rats is faster than that from anesthetized rats, reliable, reproducible, and can be used for repeated longitudinal studies.

A detailed new procedure for functional imaging of awake, head-fixed rats is described.

覚醒した頭部固定ラットのニューロイメージングのための頭部インプラント

Anesthetics, commonly used in preclinical and fundamental scientific research, have a depressive influence on the metabolic, neuronal, and vascular ...

Dual-Task Stroop Paradigm for Detecting Cognitive Deficits in High-Functioning Stroke Patients

General clinical cognitive assessment scales are not sensitive enough to cognitive impairment in high-functioning stroke patients. The dual-task assessment has advantages for identifying cognitive deficits in high-functioning stroke patients and has been gradually applied in clinical assessment and cognitive training. Moreover, the Stroop paradigm has higher sensitivity and specificity for attentional assessment than conventional clinical cognitive assessment scales. Therefore, this study presents the dual-task assessment based on the Stroop paradigm to identify cognitive deficits in high-functioning stroke patients. This study demonstrates a single- and dual-task evaluation based on the Stroop paradigm and confirms its feasibility through case experiments and synchronized functional near-infrared spectroscopy evaluation. The Stroop reaction time and correct rate are used as the main indicators to evaluate the cognitive level of the subjects. This study protocol aims to provide new ideas to figure out the ceiling effect in general clinical assessment failure for high-functioning stroke patients.

Clinical assessment scales are notsensitive enough to cognitive dysfunction in high-functioning stroke patients. The dual-task paradigm presents advantages and potential in the assessment and cognitive training of cognitive dysfunction. The study here proposes a dual-task Stroop paradigm to identify cognitive dysfunction in high-functioning stroke patients.

高機能脳卒中患者の認知障害を検出するためのデュアルタスクストループパラダイム

General clinical cognitive assessment scales are not sensitive enough to cognitive impairment in high-functioning stroke patients. The dual-task ...

Surgery and Behavioral Testing in the Tibial Neuroma Transposition Model in Rats

The tibial neuroma transposition (TNT) is a rat model in which allodynia at the neuroma site (tibial nerve) can be independently evaluated from allodynia at the plantar surface of the hind paw innervated by the intact sural nerve. This TNT model is suitable to test therapies for neuroma pain, such as the potential superiority of certain surgical therapies that are already used in the clinic, or to evaluate new drugs and their effect on both pain modalities in the same animal. In this model, a distal lesion (neurotmesis) is made in the tibial nerve, and the proximal nerve end is transposed and fixed subcutaneously and pretibially to enable assessments of the neuroma site with a 15 g Von Frey monofilament. To assess allodynia over the sural nerve, Von Frey monofilaments can be used via the up-down method on the plantar lateral region of the hind paw. After cutting the tibial nerve, mechanical hypersensitivity develops at the neuroma site within 1 week after surgery and persists at least until 12 weeks after surgery. Allodynia at the sural innervated plantar surface develops within 3 weeks after surgery compared to the contralateral limb. At 12 weeks, a neuroma forms on the proximal end of the severed tibial nerve, indicated by dispersion and swirling of axons. For the TNT model surgery, multiple critical (micro)surgical steps need to be followed, and some surgery practice under terminal anesthesia is advised. Compared to other neuropathic pain models, such as the spared nerve injury model, allodynia over the neuroma site can be independently tested from sural nerve hypersensitivity in the TNT model. However, the neuroma site can be tested only in rats, not in mice. The tips and directions provided in this protocol can help research groups working on pain successfully implement the TNT model in their facility.

This protocol describes the tibial neuroma transposition model, which entails a lesion of the tibial nerve with subsequent transposition of the proximal nerve end toward a subcutaneous pretibial or lateral position. Behavioral testing of neuroma pain and plantar hyperalgesia is quantified using Von Frey monofilaments.

ラット脛骨神経腫転位モデルにおける手術と行動検査

The tibial neuroma transposition (TNT) is a rat model in which allodynia at the neuroma site (tibial nerve) can be independently evaluated from allodynia ...

タッチ スクリーンの持続的注意課題 (土) ラット

タッチスクリーンの持続的注意課題 (土) ラット