Hot Washes, Blocking, Antibody Incubation, and Maleic Acid Buffer Washes
4:27
Probe Detection, Peroxidase Removal, and Immunofluorescence
7:19
Zebrafish Pronephros Mounting and Imaging
8:23
Results: Representative Zebrafish Pronephros and Multiciliated Cell (MCC) Imaging
9:09
Conclusion
Cilier udvikling er afgørende for korrekt organogenesis. Denne protokol beskriver en optimeret metode til at mærke og visualisere ciliated celler af zebrafisk.