Hot Washes, Blocking, Antibody Incubation, and Maleic Acid Buffer Washes
4:27
Probe Detection, Peroxidase Removal, and Immunofluorescence
7:19
Zebrafish Pronephros Mounting and Imaging
8:23
Results: Representative Zebrafish Pronephros and Multiciliated Cell (MCC) Imaging
9:09
Conclusion
Cilia er avgjørende for riktig organogenesen. Denne protokollen beskriver en optimalisert metode for å merke og visualisere tungeformet cellene i sebrafisk.