Activation of HUVEC Monolayer and Isolation/Activation of Monocytes for Transmigration
5:44
Quantification of Foam Cells and Macrophages by Microscopy or Flow Cytometry
7:42
Results: Characterizing the Transmigrating Mice
9:09
Conclusion
Transcript
The overall goal of this assay is to quantify the capacity for monocytes from human clinical samples to undergo transendothelial migration and foam cell formation. This method can be used to answer key questions in the cardiovascular field such th
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Vi beskriver en protokoll for å måle transmigration av monocytter over menneskelige endotelial monolayers og deres påfølgende modning i skum celler. Dette gir en allsidig metode å vurdere atherogenic egenskapene av monocytter isolert fra folk med ulike sykdom forhold og vurdere faktorer i blodet som kan forbedre denne tilbøyelighet.