Uniform 15N-labeled Protein Expression in BL21 ΔE3 E. coli
5:24
Purification of Recombinant Protein from E. coli
7:50
Chemical Attachment of Glucose-5-MTS to Protein by Dialysis
8:48
Results: Site-selective Cys Mutation Followed by In Vitro Glycosylation in STIM1 EF-SAM Protein
10:11
Conclusion
Transcript
The overall goal of this procedure is to incorporate cysteine residues in proteins by site directed mutagenesis at natively glycosylated sites and employ in-vitro glucose attachment and assessment approaches to evaluate the efficiency and structur
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당화 단백질의 생화학 및 구조 분석 균질 샘플 상대적으로 많은 양의 필요합니다. 여기, 우리는 효율적인 화학 반응 Cys thiols를 대상으로 박테리아에서 정화 하는 재조합 형 단백질의 사이트별 glycosylation 방법 제시.