Uniform 15N-labeled Protein Expression in BL21 ΔE3 E. coli
5:24
Purification of Recombinant Protein from E. coli
7:50
Chemical Attachment of Glucose-5-MTS to Protein by Dialysis
8:48
Results: Site-selective Cys Mutation Followed by In Vitro Glycosylation in STIM1 EF-SAM Protein
10:11
Conclusion
Transcript
The overall goal of this procedure is to incorporate cysteine residues in proteins by site directed mutagenesis at natively glycosylated sites and employ in-vitro glucose attachment and assessment approaches to evaluate the efficiency and structur
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Biokjemiske og strukturelle analyser av glycosylated proteiner krever relativt store mengder homogen prøver. Her presenterer vi en effektiv kjemisk metode for områdespesifikke glykosylering rekombinante proteiner renset fra bakterier av målretting reaktive Cys thiols.