DNA Extraction from Peripheral Blood for cfDNA and Genomics DNA (gDNA)
3:02
Library Preparation
6:47
Targeted DNA Capture
9:10
Results: Next Generation Sequencing to Detect Rare Mutations in ctDNA
10:12
Conclusion
Transcript
The overall goal of this technique is to detect mutations of low frequency in circulating tumor or ctDNA to provide clinical physicians a powerful tool to diagnose tumors and monitor tumor dynamics in response to therapy. The method of testing ctD
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Dette manuskriptet beskriver en teknikk for å oppdage mutasjoner lavfrekvente i ctDNA, ER-Seq. Denne metoden skiller seg ut med sin unike bruk av toveis feilretting, spesielle bakgrunn filter og effektiv molekylær oppnåelse.