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DOI :
10.3791/56391-v
June 1st, 2018
Chapters
0:04
Title
0:57
Grow Mouse ES Cells on Gelatin-coated Dishes
2:48
Extract DNA from Cells and Enzymatic Digestion of DNA into Single Nucleosides
5:29
Analyze 5mC and 5hmC Using UHPLC-MS/MS
8:34
Results: Methylation Status of Mouse Embryonic Stem Cells
10:17
Conclusion
我々 はマウス胚性幹細胞を培養するための 2 つの化学ベースのプロトコルについて説明します。この新しいメソッドは、(ビタミン C)、テトを介した酸化を促進し、DNA 低いメチル化とマウス胚性幹細胞の多能性を維持するために、5-メチルシトシン (PD0325901) でのデノボ合成を抑制の相乗効果のメカニズムを利用しています。
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