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DOI :
10.3791/56398-v
March 2nd, 2018
Chapters
0:04
Title
1:17
Selecting Single Cell Clones and Characterizing MeCP2 Reporter Expression
4:31
Screening the Library for Reactivation Using Hygromycin B Selection
6:44
Validating the Identified Hairpins ( Hits )
9:27
Results: Isolation of Clones with the Reporter on the Inactive X Chromosome and Characterization of Hygromycin Resistance
10:17
Conclusion
小さいヘアピン RNA (shRNA) とホタルのルシフェラーゼとマウス細胞における x 染色体不活性化のレギュレータを識別するの次世代シーケンス ベースのプロトコルについて報告して、メチル CpG 結合蛋白質 2 (hygromycin 抵抗性遺伝子融合MeCP2)不活性 X 染色体の遺伝子。
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