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DOI :
10.3791/56411-v
November 9th, 2017
Chapters
0:05
Title
0:56
Growth and Collection of Vegetative D. discoideum
2:51
Acute Mechanical Stimulation Using an Orbital Shaker
4:18
Acute Mechanical Stimulation Using a Microfluidic Chamber
6:33
Applying Pharmacological Treatments Using the Microfluidic Chamber
7:48
Results: Observed Shear Force Responses in D. discoideum
9:18
Conclusion
在这里, 我们描述了评估急性机械刺激的细胞反应的方法。在 microscopy-based 试验中, 我们研究了荧光标记的生物传感器的局限性, 并对剪切流进行了简单的刺激。我们还测试了各种感兴趣的蛋白质的活化反应的急性机械刺激生化。
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