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DOI :
10.3791/56411-v
November 9th, 2017
Chapters
0:05
Title
0:56
Growth and Collection of Vegetative D. discoideum
2:51
Acute Mechanical Stimulation Using an Orbital Shaker
4:18
Acute Mechanical Stimulation Using a Microfluidic Chamber
6:33
Applying Pharmacological Treatments Using the Microfluidic Chamber
7:48
Results: Observed Shear Force Responses in D. discoideum
9:18
Conclusion
ここで我々 は急性の機械的刺激に対する細胞応答を評価するための方法を説明します。顕微鏡ベースのアッセイでは、蛍光標識したバイオ センサー シアー流を含む簡単な刺激の局在を調べる。我々 はまた、生化学的急性の機械的刺激に対する応答の興味の様々 な蛋白質の活性化をテストします。
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