Lentiviral Transduction and Stable Line Generation
4:53
Dose Response Effect of Proteosome Inhibitors on Protein Aggregation and Image Analysis
7:06
Time lapse Imaging of YFP Expression in Living Cells
8:09
Results: Quantification of SOD1 Mutant Protein Aggregate Formation
10:04
Conclusion
Transcript
The overall goal of this experiment is to develop an assay for studying the effect of small molecules in modulating the aggregation of misfolded proteins in a time and dose dependent manner. This method can help answer the key questions for develo
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Vi beskriver en metode til at kvantificere sammenlægning af fejlfoldede proteiner. Vores lentiviral protokoloplysninger induceret stabil celle linje generation, automatiseret Konfokal imaging og billedanalyse af protein aggregater. Som et vejledende program undersøgte vi effekten af små molekyler i at fremme SOD1 akkumulering i en tid - og dosisafhængig måde.