Cardiac Crescent Stage Embryo Harvest and Processing
2:45
Immunofluorescence Staining
3:57
Embryo Mounting and Confocal Imaging
5:28
Image Analysis and Quantitative 3D Modeling
7:49
Results: Representative Confocal Image Processing
8:56
Conclusion
Transcript
The overall goal of this method is to quantitatively assess cardiac progenitor cell localization and distribution during heart organogenesis. This method can help answer key answers in the heart development field by allowing us to observe the loca
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Her beskriver vi en protokoll for hele mount immunofluorescence og bildebasert kvantitative volumetric analyse av tidlig stadium mouse embryoer. Vi presenterer denne teknikken som en kraftig tilnærming til kvalitativt og kvantitativt vurdere cardiac strukturer under utvikling, og foreslår at det kan være mye tilpasses andre organsystemer.