Invasion Assay Using 3D Matrices

The extracellular matrix (ECM) is a network of molecules that provide a structural framework for cells and tissues and helps facilitate intercellular communication. Three-dimensional cell culture techniques have been developed to more accurately model this extracellular environment for in vitro study. While many cell processes during migration through 3D matrices are similar to those required for movement across rigid 2D surfaces, including adherence, migration through ECM also requires cells to modulate and invade this polymeric-mesh of ECM.

In this video, we will present the structure and function of ECM and the basic mechanisms of how cells migrate through it. Then, we will examine the protocol of an assay for tube formation by endothelial cells, whose steps can be generalized to other experiments based on 3D matrices. We will finish by exploring several other biological questions that can be addressed using ECM invasion assays.

Scientists have developed 3D models to more accurately study cell invasion and migration processes. While most traditional cell culture systems are 2D, cells in our tissues exist within a 3D network of molecules known as the extracellular matrix or ECM. While many of the mechanistic processes required for cell motility in 2D and 3D are similar, factors such as the reduced stiffness of ECM compared to plastic surfaces, the addition of a third dimension for migration, and the physical hindrance of moving through the mesh of

Explore More Videos

Extracellular Matrix ECM

Cell Motility

ECM Composition

Endothelial Cell Invasion

Biological Questions