February 4th, 2018
•Nested PCR is a sensitive, specific, and straightforward technique that can be applied to tick DNA extracts to probe for Borrelia burgdorferi, the causative agent of Lyme disease. The initial PCR experiment uses gene-specific primers to generate long amplicons, which then become templates for a subsequent reaction using internal primers.
Related Videos
Quantitative Real-Time PCR using the Thermo Scientific Solaris qPCR Assay
Analysis of Gene Expression in Emerald Ash Borer (Agrilus planipennis) Using Quantitative Real Time-PCR
Rapid PCR Thermocycling using Microscale Thermal Convection
Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides
Genome-wide Gene Deletions in Streptococcus sanguinis by High Throughput PCR
In Vivo Modeling of the Morbid Human Genome using Danio rerio
Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution Melt Analysis
Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
Detecting Estrogenic Ligands in Personal Care Products using a Yeast Estrogen Screen Optimized for the Undergraduate Teaching Laboratory
The MUB40 Peptide for Use in Detecting Neutrophil-Mediated Inflammation Events
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved