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JoVE Journal

Neuroscience

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Focal Macropatch Recordings of Synaptic Currents from the Drosophila Larval Neuromuscular Junction

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07:01 min

September 25th, 2017

September 25th, 2017

5,984 Views

0:05

Title

0:39

Fabrication of Recording Electrodes

1:53

Additional Preparation

3:17

EJC Electrical Recording

4:33

EJC Electrical Stimulation and Seal Resistance

5:34

Results: Accurate Detection of Release Events

6:29

Conclusion

Transcript

The overall goal of this technique is to monitor synaptic activity of visualized synaptic boutons at the drosophila larval neuromuscular junction. An excellent model system readily amendable to genetic manipulations. This technique can address key

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Synaptic currents can be recorded focally from visualized synaptic boutons at the Drosophila third instar larvae neuromuscular junction. This technique enables monitoring the activity of a single synaptic bouton.

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