Isolation of Human Peripheral Blood Mononuclear Cells
2:00
Tetramer-Associated Magnetic Enrichment of Antigen Specific B-Cells
3:58
Staining of Antigen Specific Human B-Cells and Cell Sorting
4:56
Single Cell RT-PCR
6:00
Screening and Verification of Heavy and Light Chain Expressing Vectors
7:45
Production of Monoclonal Antibodies
10:33
Results: Detection of Relevant Antigen Specific B-Cells and Generation of Highly Discriminative Monoclonal Antibody from Donor Peripheral Blood Mononuclear Cells
12:41
Conclusion
Transcript
The overall goal of this procedure is to produce fully human monoclonal antibodies against a desired antigen starting from human B lymphocytes circulating in the blood. This protocol allows the generation of monoclonal antibodies that can discrimi
Sign in or start your free trial to access this content
Descriviamo un metodo per la produzione di anticorpi umani specifici per un antigene di interesse, a partire da rari linfociti B circolanti nel sangue umano. Generazione di questi anticorpi naturali è efficiente e rapido, e gli anticorpi ottenuti in grado di discriminare tra antigeni altamente correlati.