Isolation of Human Peripheral Blood Mononuclear Cells
2:00
Tetramer-Associated Magnetic Enrichment of Antigen Specific B-Cells
3:58
Staining of Antigen Specific Human B-Cells and Cell Sorting
4:56
Single Cell RT-PCR
6:00
Screening and Verification of Heavy and Light Chain Expressing Vectors
7:45
Production of Monoclonal Antibodies
10:33
Results: Detection of Relevant Antigen Specific B-Cells and Generation of Highly Discriminative Monoclonal Antibody from Donor Peripheral Blood Mononuclear Cells
12:41
Conclusion
Transcript
The overall goal of this procedure is to produce fully human monoclonal antibodies against a desired antigen starting from human B lymphocytes circulating in the blood. This protocol allows the generation of monoclonal antibodies that can discrimi
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Vi beskriver en metod för framställning av humana antikroppar specifika för antigen av intresse, start från sällsynta B-celler som cirkulerar i blod. Generation av dessa naturliga antikroppar är snabbt och effektivt, och antikroppar erhållits kan diskriminera mellan starkt relaterade antigener.