Coating of Bacteria with NHS-biotin/Streptavidin-conjugated Magnetic Beads
4:38
Bone Marrow-derived Macrophages (BMDM) Infection
6:18
Isolation of S. typhimurium-containing Phagosomes
8:21
Results: Successful Biotinylation of Bacterial and Isolation of Bacterial-containing Phagosomes
9:41
Conclusion
Transcript
The overall goal of this procedure is to isolate intact and enriched bacteria containing Phagosomes. This method can help us answer key questions in the field of Immunology such as changes in Phagosome maturation and antigen processing when pathog
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Biz burada Salmonella typhimuriumyalıtım için basit ve hızlı bir yöntem tarif-biotin ve streptavidin bakterilerle kaplama tarafından phagosomes üzerinden makrofajlar içeren.