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DOI :
10.3791/56550-v
December 20th, 2017
Chapters
0:05
Title
0:35
Site-directed Mutagenesis
1:45
Transformation and Screening for the Desired Clone
2:35
pcDNA3.1/GLA and pcDNA3.1/GAA Plasmid Transfection and Treatment of HEK293H
4:00
Cell Harvest
4:43
Homogenization by Freezing and Thawing and BCA Assay
6:17
Enzyme Activity Measurement with Artificial 4-Methylumbeliferyl Substrate
8:19
Results: Enzyme Activity Measurement Mutagenized 3 α-galactosidase and 3 Acid α-glucosidase
9:35
Conclusion
有一个要求, 使临床前测试的一个新的类 "孤儿" 药物称为药理伴侣可再生, 快速, 高效。我们开发了一个简单, 高度标准化, 多功能细胞文化检测, 以筛选合格的病人以及新的药理陪护药物。
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