Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Please note that all translations are automatically generated. Click here for the English version.
DOI :
10.3791/56550-v
December 20th, 2017
Chapters
0:05
Title
0:35
Site-directed Mutagenesis
1:45
Transformation and Screening for the Desired Clone
2:35
pcDNA3.1/GLA and pcDNA3.1/GAA Plasmid Transfection and Treatment of HEK293H
4:00
Cell Harvest
4:43
Homogenization by Freezing and Thawing and BCA Assay
6:17
Enzyme Activity Measurement with Artificial 4-Methylumbeliferyl Substrate
8:19
Results: Enzyme Activity Measurement Mutagenized 3 α-galactosidase and 3 Acid α-glucosidase
9:35
Conclusion
再現性の高い、高速、かつ効率的に薬理学的シャペロンと呼ばれる「オーファン」薬の新規クラスのテスト前臨床をする需要があります。新規の薬理学的シャペロン薬と同様に、対象となる患者の画面に、高度に標準化、シンプルで汎用性の高い細胞培養技術を用いた試験を行った。
Tags
-- Views
Related Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Overview
Publishing Process
Editorial Board
Scope and Policies
Peer Review
FAQ
Submit
Librarians
Testimonials
Subscriptions
Access
Resources
Library Advisory Board
ABOUT JoVE
Leadership
Copyright © 2024 MyJoVE Corporation. All rights reserved