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DOI :
10.3791/56550-v
December 20th, 2017
Chapters
0:05
Title
0:35
Site-directed Mutagenesis
1:45
Transformation and Screening for the Desired Clone
2:35
pcDNA3.1/GLA and pcDNA3.1/GAA Plasmid Transfection and Treatment of HEK293H
4:00
Cell Harvest
4:43
Homogenization by Freezing and Thawing and BCA Assay
6:17
Enzyme Activity Measurement with Artificial 4-Methylumbeliferyl Substrate
8:19
Results: Enzyme Activity Measurement Mutagenized 3 α-galactosidase and 3 Acid α-glucosidase
9:35
Conclusion
전 임상 재현, 빠르고, 효율적인 약리 보호자를 호출 하는 "고아" 약물의 소설 클래스에 대 한 테스트 있도록 수요가 있다. 우리 소설 약리 보호자 약물으로 서 적격 환자에 대 한 간단 하 고, 고도로 표준화, 다양 한 세포 문화 기반 분석 결과 화면을 개발 했다.
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