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JoVE Journal

Neuroscience

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Visualization of Thalamocortical Axon Branching and Synapse Formation in Organotypic Cocultures

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06:16 min

March 28th, 2018

March 28th, 2018

6,391 Views

0:04

Title

0:58

Preparation of Cortical Slices

2:24

Procedure of Electroporation

4:37

Results: Thalamocortical Axons Coculture and Time-lapse Imaging

5:38

Conclusion

Transcript

The overall goal of this method is to study dynamic morphological changes of axon branching and synapse formation in the thalamocortical projection. This method can help answer key questions in the field of neurobiology, such as new neural circuit

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This protocol describes a method for simultaneous imaging of thalamocortical axon branching and synapse formation in organotypic cocultures of the thalamus and cerebral cortex. Individual thalamocortical axons and their presynaptic terminals are visualized by a single cell electroporation technique with DsRed and GFP-tagged synaptophysin.

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