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DOI :
10.3791/56553-v
March 28th, 2018
Chapters
0:04
Title
0:58
Preparation of Cortical Slices
2:24
Procedure of Electroporation
4:37
Results: Thalamocortical Axons Coculture and Time-lapse Imaging
5:38
Conclusion
本协议描述了丘脑和大脑皮层脊髓 cocultures 中丘脑-皮质轴突分支和突触形成同时成像的方法。单个丘脑-皮质轴突及其突触前终端的可视化细胞电穿孔技术与 DsRed 和 GFP 标记突触素。
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