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DOI :
10.3791/56553-v
March 28th, 2018
Chapters
0:04
Title
0:58
Preparation of Cortical Slices
2:24
Procedure of Electroporation
4:37
Results: Thalamocortical Axons Coculture and Time-lapse Imaging
5:38
Conclusion
このプロトコルでは、視床皮質軸索分岐およびシナプス形成の脊髄共培養系における視床と大脳皮質の同時イメージング法について説明します。個々 の視床皮質軸索とその神経終末は、下流と GFP タグ シナプトフィジン単細胞エレクトロポレーション法による可視化します。
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