The overall goal of this fluorescence imaging approach is to determine the half-life of a protein of interest in single living cells using a SNAP-tag. Cellular proteins are turned over extensively with synthesis and degradation rates being specifi
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Dit protocol beschrijft een methode om te bepalen eiwit halfwaardetijd in één levende Adherente cellen, met behulp van pulse labeling en fluorescentie time-lapse beeldvorming van module-tag fusion eiwitten.