The overall goal of this fluorescence imaging approach is to determine the half-life of a protein of interest in single living cells using a SNAP-tag. Cellular proteins are turned over extensively with synthesis and degradation rates being specifi
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Denne protokollen beskriver en metode for å bestemme protein halv-bor i enkelt levende tilhenger celler, puls merking og fluorescens tidsinnstilt bildebehandling SNAPIN-tag fusion proteiner.