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Single-Cell Quantification of Protein Degradation Rates by Time-Lapse Fluorescence Microscopy in Adherent Cell Culture

8.5K Views

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12:06 min

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February 4th, 2018

DOI :

10.3791/56604-v

February 4th, 2018

•
8,458 Views
1UPSUTER, Institute of Bioengineering (IBI), School of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL)

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Keywords Protein Degradation
Single cell
Time lapse Fluorescence Microscopy
Adherent Cell Culture
SNAP tag
Protein Half life
Fluorescence Imaging
Protein Turnover
Heterogeneity
Endogenous Protein Tagging
Overexpression
DNA Repair Enzyme
Benzylguanine
Fluorescent Dye
Exponential Decay
Embryonic Stem Cells
Monolayer
Cell cell Interactions
N cadherin
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