Mononuclear Cells Isolation by Density Gradient Centrifugation
5:26
Cytospin Preparation, γH2AX and 53BP1 Immunofluorescence Staining, and Analysis
8:46
Results: Clearly Visible Foci and DNA Repair
10:02
Conclusion
Transcript
The overall goal of gamma-H2AX and 53BP1 immunofluorescence microscopy is to analyze the formation and repair of DNA double-strand breaks in various tissues. This method can help answer key questions in cancer research, such as how genetic instabi
Sign in or start your free trial to access this content
Ce manuscrit fournit un protocole pour l’analyse des cassures double brin de l’ADN par la microscopie en immunofluorescence de γH2AX et 53BP1.