Collecting NPCs for Plating of Experimental Conditions
2:30
Neural Precursor Cell DNA Synthesis Assay
3:47
S Phase Entry Assay
4:39
Neural Precursor Cell Number Assay
5:41
Neural Precursor Cell Neurosphere Migration Assay
7:58
Results: Use of Phase-contrast and Fluorescence Microscopy to Study the Proliferation, Migration, and Neurite Outgrowth of Neural Precursor Cells
9:51
Conclusion
Transcript
The overall goal of this protocol is to characterize early brain developmental processes of human neural precursor cells in a rapid and reproducible fashion, and to define the effects of important extracellular factors in regulating these processe
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Neurologische processen zoals proliferatie, migratie en neurite uitvloeisel zijn vaak verstoord in neuropsychiatrische ziekten. Dus presenteren we protocollen om te snel en reproducibly beoordelen deze neurologische processen in menselijke iPSC afkomstige NPC's. Deze protocollen toestaan ook de beoordeling van de gevolgen van relevante factoren die de groei en therapeutics NPC ontwikkelingssamenwerking.