Collecting NPCs for Plating of Experimental Conditions
2:30
Neural Precursor Cell DNA Synthesis Assay
3:47
S Phase Entry Assay
4:39
Neural Precursor Cell Number Assay
5:41
Neural Precursor Cell Neurosphere Migration Assay
7:58
Results: Use of Phase-contrast and Fluorescence Microscopy to Study the Proliferation, Migration, and Neurite Outgrowth of Neural Precursor Cells
9:51
Conclusion
Transcript
The overall goal of this protocol is to characterize early brain developmental processes of human neural precursor cells in a rapid and reproducible fashion, and to define the effects of important extracellular factors in regulating these processe
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Neurodevelopmental prosesser som spredning, migrering og neurite resultatet er ofte opprørt i nevropsykiatriske sykdommer. Derfor presenterer vi for å raskt og reproduserbar vurdere prosessene neurodevelopmental i menneskelig iPSC-avledet NPCer. Disse protokollene kan også vurdering av virkningene av relevante vekstfaktorer og therapeutics på NPC-utvikling.