Transfection of HEK293 Cells with NR1-GFP Expression Plasmid
2:38
Cell-based Immunofluorescence Assay
4:41
Results: Anti-NMDA Receptor Autoantibodies Detected in the Blood of Patients with Autoimmune Encephalitis
6:12
Conclusion
Transcript
The overall goal of this assay is to detect autoantibodies against the NMDA receptor in the blood of patients with suspected autoimmune encephalitis. The measure help answer the key questions in differential diagnosis of patients with acute neuro-
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Hemos expresado ectopically subunidad NR1 del receptor NMDA etiquetada con proteína verde fluorescente en células embrionarias humanas (HEK293) como antígeno para detectar autoanticuerpos contra el receptor de NMDA en la sangre de pacientes con encefalitis autoinmune. Este sencillo método puede ser adecuado para la detección de propósitos en contextos clínicos.