Transfection of HEK293 Cells with NR1-GFP Expression Plasmid
2:38
Cell-based Immunofluorescence Assay
4:41
Results: Anti-NMDA Receptor Autoantibodies Detected in the Blood of Patients with Autoimmune Encephalitis
6:12
Conclusion
Transcript
The overall goal of this assay is to detect autoantibodies against the NMDA receptor in the blood of patients with suspected autoimmune encephalitis. The measure help answer the key questions in differential diagnosis of patients with acute neuro-
Sign in or start your free trial to access this content
Vi uttryckte ectopically NR1-subenheten av NMDA-receptorn taggade med grönt fluorescerande protein i mänskliga embryonala celler (HEK293) som antigen att upptäcka autoantikroppar mot NMDA-receptorn i blodet hos patienter med misstänkt med autoimmun encefalit. Denna enkla metod kan vara lämplig vid screening i kliniska inställningar.