Bone Marrow Macrophage Derivation and Activation with Antibodies: In Vitro Testing
5:34
Harvesting Peritoneal Macrophages after an IVIg + LPS Challenge: In Vitro Testing
8:38
Results: BMDM Reaction to IVIg + LPS Treatment
10:06
Conclusion
Transcript
The overall goal of these experiments is to determine the effects of antibody-based drugs on anti-inflammatory macrophage activation states using mouse bone marrow and peritoneal macrophages. This method can help answer key questions in the field
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Antibody-based drugs have revolutionized treatment for inflammatory diseases. In addition to having direct effects on specific targets, antibodies can activate macrophages to become anti-inflammatory. This protocol describes how anti-inflammatory macrophage activation can be assessed in vitro, using mouse bone marrow macrophages, and in vivo, using peritoneal macrophages.