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DOI :
10.3791/56693-v
February 22nd, 2018
Chapters
0:04
Title
0:52
Insect Cell Culture
1:53
Transient Protein Expression
5:00
Anti-apoptotic Activity Assay
7:04
Cell Viability Assay
8:11
Results: Transient Protein Expression Enables Analysis of Anti-apoptotic Activity
9:41
Conclusion
本协议描述了一种热休克诱导蛋白表达系统 (pDHsp/V5-His/sf9 细胞系统), 可用于表达外来蛋白或评价潜在外来蛋白的抗凋亡活性及其截断的氨基酸昆虫细胞中的酸。
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