Ligation of Annealed Oligos into Digested Backbone
3:02
Generation of Cell Lines Stably Expressing spCas9
5:40
Reporter Assay for Cas9 Activity
6:10
Spinfection of sgRNAs into Cas9 Expressing Cells
6:31
Ba/F3 Cellular Transformation and Positive Selection using m-IL3 Withdrawal
7:23
Results: Use of CRISPR/Cas9 Tool and Ba/F3 Cellular Transformation to Study Oncogenic Potential of Mutated Calreticulin
9:02
Conclusion
Transcript
The overall goal of this experiment is to utilize CRISPR/Cas9 gene editing to model calreticulin mutations in cytokine dependent hematopoietic cells in order to study their oncogenic activity. This method can help answer key questions in the hemat
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타겟된 유전자 CRISPR/Cas9를 사용 하 여 편집 크게 유전자의 생물 학적 기능에 대 한 이해를 촉진 했다. 여기, 우리가 그들의 종양 활동을 공부 하기 위하여 모델 calreticulin 돌연변이 cytokine 종속 조 혈 모 세포에서에 CRISPR/Cas9 방법론을 활용 합니다.